The complex and exciting scenario of the neurodegenerative diseases illustrated by Dr. Marco Peviani at our Lab.
The neuroinflammatory processes contribute to shaping the neuronal microenvironment and have a direct impact on the disease progression. Accumulated evidences suggest that the heterogenous glial cell responses occurring during disease progression
reflect not only the extent of neuronal demise in different CNS regions, but also variable engagement in the attempts to cope with the neuronal damage.
He has presented his current efforts ranging from single-cell RNAseq profiling to development of novel MRI/PET traceable cell-targeted nanovector platforms to obtain better insights into the complexity of glial-cell responses and possibly pave the way for more efficacious therapeutic approaches.
We are working on the orthogonal chemical functionalization of colloidal nanoparticles (nanogels) through surface decoration with different moieties, based on peptide sequences or specific chemical groups, to promote the selective interaction with target cells. Indeed, the in vivo application of these biomaterials is strictly linked to their capability to selectively interact with specific inflamed, damaged or malignant cells, avoiding the immune response and the consequent undesired macrophage uptake.
Project ITHACa - granted by intramural funds in the framework of INTESE Project (FILAS-RU-2014-1193) - aims to achieve seamless hw and sw integration between microfluidic cell-on-a-chip devices and high content screening microscopy.
Industrial Partners of ITHACa Consortium are Nikon Instruments SpA and BioNova Technologies.
Our NAFLD-on-a-chip model represents an advanced in vitro model to reproduce the early onset of NonAlcoholic Fatty Liver Disease (NAFLD) in a liver-on-a-chip microfluidic format.
In collaboration with Dr. Filippo Rossi (Politecnico di Milano, Italy) we are studying the potential of nanomedicine products in the management of NAFLD. In the video, a representative 3D stack of hepatic cells actively uptaking the nanovectors (in red).
Our publication on Scientific Reports discloses the computationally informed design and the experimental validation of an actuated microfluidic device for the delivery of biaxial strain patterns on cultured cells.
By coupling a microfluidic printing head with a gantry motorized system, researchers at the Tissue Engineering Lab, Università Campus Bio-Medico were able to print skeletal myoblasts in a 3D fashion, leading to increased myogenesis and myotube alignment.
The improved resolution guaranteed by the microfluidic printing head was essential to improve cell alignment and myotube formation.
The study, performed in the framework of an international collaboration, have been published on Biomaterials.
UCBM has hosted the workshop "Innovation in biomedicine: advanced in vitro and in silico models", a joint initiative of the following projects: RF-2011/12 (#02347120), PRIN-2012 (#20125NMMLA), and UCBM Intramural Research Grant.
The smart integration of cell culture protocols, tissue engineering, microfabrication, advanced microscopy, and computational tools is leading to a new generation of research tools for diverse applications ranging from drug discovery to precision medicine. Additionally, in vitro and in silico models may represent a valid alternative to animal experimentation, fulfilling the 3Rs paradigm.
Below, a picture of the organizers and the invited speakers.